{"id":334426,"date":"2018-09-11T19:40:39","date_gmt":"2018-09-11T19:40:39","guid":{"rendered":"https:\/\/www.sentryair.com\/blog\/?p=334426"},"modified":"2021-02-10T19:28:56","modified_gmt":"2021-02-10T19:28:56","slug":"mycology-culture-sampling-testing","status":"publish","type":"post","link":"https:\/\/www.sentryair.com\/blog\/industry-applications\/laboratories\/mycology-culture-sampling-testing\/","title":{"rendered":"Mycology Culture Sampling & Testing"},"content":{"rendered":"
\"Ductless<\/a>

Micrograph of a fungal organism Exophiala Salmonis. (Source)<\/a><\/p><\/div>\n

Mycology, a branch of biology, focuses on the study of fungi. Heinrich Anton de Bary, a research botanist from Germany in the 1800s, is credited for his ground-breaking research on fungi-related plant diseases, which laid the foundation for modern mycology and plant pathology.<\/p>\n

The field of mycology is vast and includes a wide array of research focuses, including:<\/em>
\n\u2022 Medical and Pharmacological Research
\n\u2022 Fungal Genetics
\n\u2022 Fungal Infections and Diseases (in plants, humans, and animals)
\n\u2022 Agriculture and Environmental Uses
\n\u2022 Food and Beverage (Beer, Wine, Bread, Yeast, etc.)<\/p>\n

Some of the more notable contributions that mycological research has provided are the development of antibiotic and cholesterol-lowering drugs including penicillin, streptomycin, and tetracycline.
\n 
\n 
\n <\/p>\n

Sampling and Testing<\/h2>\n
\"Ductless<\/a>

Leaf Specimen with Fungi (Source)<\/a><\/p><\/div>\n

When studying fungi, mycologists take several samples for testing and research. Depending on the area of study, samples may include:<\/p>\n

\u2022 Plants and Mushrooms
\n\u2022 Mold
\n\u2022 Nails
\n\u2022 Hair
\n\u2022 Skin
\n\u2022 DNA
\n\u2022 Urine
\n\u2022 Tissue Biopsies<\/p>\n

These samples are then tested and analyzed to determine the type of fungus present as well as its effect (positive or negative) on the host sample.<\/p>\n

It is important for researchers and laboratory technicians to guard their respiratory health when handling these samples; particularly those in the field of fungal infection and disease research. Microscopic fungal strains, as well as human or animal hair, urine, tissue, and DNA may pose a respiratory risk if inhaled during processing and analysis.<\/p>\n

Recommended Laboratory Safety Standards<\/h2>\n

According to the Center for Disease Control\u2019s Guidelines for Safe Work Practices in Human and Animal Medical Diagnostic Laboratories<\/em>, it is \u201crecommended that mycology laboratories that culture for filamentous fungi and manipulate those organisms be separate and isolated from the main microbiology laboratory with negative air pressure moving into the room from the main laboratory.\u201d (CDC)<\/p>\n

The Guideline further states that \u201cevery diagnostic microbiology laboratory needs one or more BSC as a primary means of containment for working safely with infectious organisms.\u201d In chapter 3 of the Guideline, the CDC refers to using HEPA filters that remove at least 99.97% of 0.3 \u00b5m particles. The use of a negative-pressure work zone, coupled with a HEPA filter, is a proven safety measure that helps protect workers from inhalation exposure to hazardous-laden microscopic particles. Ductless Fume Hoods and Portable Clean Rooms would both provide safety measures to protect the user and environment from harmful mycology samples.<\/p>\n

Portable, Ductless Fume Hoods for Laboratory Workstations<\/h2>\n

Ductless Fume Hoods, also known as Ductless Containment Hoods, are self-contained negative-pressure workstations that help protect the operator’s breathing zone from harmful airborne fumes and particulates.
\n 
\n\"Ductless<\/a><\/p>\n

\n1. Ambient Air is drawn into the hood and mixed with fumes, vapors or particulates emitted from the process.
\n 
\n2. The air within the hood is processed through a pre-filter where some particles are captured.
\n 
\n3. Air is filtered through the main filter (HEPA, ULPA, ASHRAE, Activated Carbon or Specialty Blended).
\n 
\n4. Filtered air is released back into the room eliminating the need for ductwork.<\/div>\n
<\/div>\n

 
\nUtilizing a powerful fan, ambient air is pulled in through the hood\u2019s inlet and into a filtration system. The filters work to keep harmful airborne contaminants away from the operator’s respiratory zone while they work within the hood. Because the air is recirculated, no exterior-ducting or loss of heat and air conditioning is necessary when using a fume hood.<\/p>\n

\"Sentry<\/a><\/p>\n

Sentry AirHawk<\/strong> \u2014 Model # AirHawk30 <\/a><\/h3>\n

Features:<\/strong> Multi-level Filter Chamber*, Variable Speed Control, and Dimmable 1400 Lux LED Light
\n*If solvents are being utilized, the filter chamber will have an optional activated carbon filter.<\/p>\n

Approx. Inlet Velocity: <\/strong>
\nPre-filter & 99.97% HEPA Filter – 130 FPM High; 25 FPM Low
\nPre-filter & Heavy-duty Carbon Filter – 115 FPM High; 20 FPM Low<\/p>\n

Multiple widths available:<\/strong> 30″ (shown), 40″, 50″<\/p>\n

For additional visibility, the hood can be made of all clear PVC.<\/p>\n

<\/div>\n
\n

\"330<\/a><\/p>\n

30\u201d and 40\u201d Wide Ductless Fume Hood<\/strong> \u2014
\nModel # SS-330-DCH<\/a> and SS-340-DCH<\/a><\/h3>\n

Features:<\/strong> Variable Speed Control and a Fluorescent Light<\/p>\n

Approx. Inlet Velocity: <\/strong>
\nPre-filter & 99.97% HEPA Filter – 130 FPM High; 25 FPM Low
\nPre-filter & Heavy-duty Carbon Filter – 115 FPM High; 20 FPM Low<\/p>\n

Multiple widths available: <\/strong>
\n12″, 18″, 24″, 30\u201d (shown), 40′, 50″, 60″, 70″<\/p>\n

For additional visibility, a transparent version is available at extra cost.
\n 
\n 
\n <\/p>\n

\n

Utilizing Positive Pressure for Laboratory Work<\/h2>\n

There are some instances during mycology work where samples need to be kept under positive pressure in order to not cross contaminate. For these applications, a Portable Clean Room Hood (PCR) is an excellent solution.<\/p>\n

\"PCR<\/a>
\n1. Room air is pulled into the air cleaner.
\n 
\n2. Air is processed through a MERV 8 Pre-Filter, where some particles are absorbed.
\n 
\n3. Air is filtered by passing through a final HEPA (up to 99.97% efficiency on particles as small as 0.3 microns) or ULPA (up to 99.9997% efficiency on particles as small as 0.12 microns) Filter.
\n 
\n4. Filtered air is discharged into the work area of the hood in a vertical laminar flow pattern, creating a particle-free enclosure.<\/p>\n

<\/div>\n
\n

 
\n\"18<\/a><\/p>\n

18\u201d Wide Portable Clean Room Hood<\/strong> \u2014
\nModel # SS-218-PCR<\/a><\/h3>\n

All of Sentry Air\u2019s PCRs have an ISO Class 5 rating, utilize a dual-stage filtration process consisting of a pre-filter and a HEPA or ULPA filter, and come in a variety of sizes. The 18\u201d PCR in particular, can be easily integrated into existing workspaces because of its size and can be transported to various locations without difficulty because of its lightweight and portable nature.<\/p>\n

<\/div>\n
\"Sentry<\/p>\n

For More Information, Please Contact Us!<\/em><\/h4>\n

1.800.799.4609
\nsales@sentryair.com<\/a><\/span>
\nwww.sentryair.com<\/a><\/span><\/p>\n<\/div>\n

Sources<\/h2>\n

\u2022 WiseGeek \u2013 What Does a Mycologist Do? https:\/\/www.wisegeek.com\/what-does-a-mycologist-do.htm<\/a>
\n\u2022 Wikipedia \u2013 Mycology, https:\/\/en.wikipedia.org\/wiki\/Mycology<\/a>
\n\u2022 Britannica \u2013 Mycology, https:\/\/www.britannica.com\/science\/mycology<\/a>
\n\u2022 Britannica – Heinrich Anton de Bary, https:\/\/www.britannica.com\/biography\/Heinrich-Anton-de-Bary#ref232082 <\/a>
\n\u2022 CDC \u2013 Guidelines for Safe Work Practices in Human and Animal Medical Diagnostic Laboratories, https:\/\/www.cdc.gov\/mmwr\/pdf\/other\/su6101.pdf<\/a> (Chapters 3 and 7)<\/p>\n","protected":false},"excerpt":{"rendered":"

Mycology, a branch of biology, focuses on the study of fungi. Heinrich Anton de Bary, a research botanist from Germany in the 1800s, is credited for his ground-breaking research on fungi-related plant diseases, which laid the foundation for modern mycology and plant pathology. The field of mycology is vast and includes a wide array of… Learn More<\/a><\/p>\n","protected":false},"author":2,"featured_media":334427,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[84,1059,1006],"tags":[],"class_list":["post-334426","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-ductless-fume-hood","category-laboratories","category-portable-clean-room"],"yoast_head":"\nMycology Culture Testing & Sampling | Fume Hoods & Portable Clean Rooms<\/title>\n<meta name=\"description\" content=\"When studying mycology, the study of fungi, scientists must utilize safety control devices such as a ductless fume hoods or portable clean rooms.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.sentryair.com\/blog\/industry-applications\/laboratories\/mycology-culture-sampling-testing\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Mycology Culture Testing & Sampling | Fume Hoods & Portable Clean Rooms\" \/>\n<meta property=\"og:description\" content=\"When studying mycology, the study of fungi, scientists must utilize safety control devices such as a ductless fume hoods or portable clean rooms.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.sentryair.com\/blog\/industry-applications\/laboratories\/mycology-culture-sampling-testing\/\" \/>\n<meta property=\"og:site_name\" content=\"Sentry Air Systems, Inc.\" \/>\n<meta property=\"article:published_time\" content=\"2018-09-11T19:40:39+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2021-02-10T19:28:56+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.sentryair.com\/blog\/wp-content\/uploads\/2018\/09\/Exophiala_salmonis_CDC.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"355\" \/>\n\t<meta property=\"og:image:height\" content=\"454\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"sentryair\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"sentryair\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"5 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.sentryair.com\/blog\/industry-applications\/laboratories\/mycology-culture-sampling-testing\/\",\"url\":\"https:\/\/www.sentryair.com\/blog\/industry-applications\/laboratories\/mycology-culture-sampling-testing\/\",\"name\":\"Mycology Culture Testing & Sampling | Fume Hoods & Portable Clean Rooms\",\"isPartOf\":{\"@id\":\"https:\/\/www.sentryair.com\/blog\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.sentryair.com\/blog\/industry-applications\/laboratories\/mycology-culture-sampling-testing\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/www.sentryair.com\/blog\/industry-applications\/laboratories\/mycology-culture-sampling-testing\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.sentryair.com\/blog\/wp-content\/uploads\/2018\/09\/Exophiala_salmonis_CDC.jpg\",\"datePublished\":\"2018-09-11T19:40:39+00:00\",\"dateModified\":\"2021-02-10T19:28:56+00:00\",\"author\":{\"@id\":\"https:\/\/www.sentryair.com\/blog\/#\/schema\/person\/799b2bc2f157a0b1c3ef4121378f9ee5\"},\"description\":\"When studying mycology, the study of fungi, scientists must utilize safety control devices such as a ductless fume hoods or portable clean rooms.\",\"breadcrumb\":{\"@id\":\"https:\/\/www.sentryair.com\/blog\/industry-applications\/laboratories\/mycology-culture-sampling-testing\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.sentryair.com\/blog\/industry-applications\/laboratories\/mycology-culture-sampling-testing\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.sentryair.com\/blog\/industry-applications\/laboratories\/mycology-culture-sampling-testing\/#primaryimage\",\"url\":\"https:\/\/www.sentryair.com\/blog\/wp-content\/uploads\/2018\/09\/Exophiala_salmonis_CDC.jpg\",\"contentUrl\":\"https:\/\/www.sentryair.com\/blog\/wp-content\/uploads\/2018\/09\/Exophiala_salmonis_CDC.jpg\",\"width\":355,\"height\":454,\"caption\":\"Micrograph of a fungal organism Exophiala Salmonis. 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